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. 2019 Mar 27;67(7):523–535. doi: 10.1369/0022155419841013

Figure 2.

Figure 2.

Rho-kinase inhibitor induces primary cilia elongation in undifferentiated mouse cochlear precursors. Images showing double-immunocytostaining for Ac-tubulin (red) and γ-tubulin (green) of undifferentiated US/VOT-E36 cells treated with Rho-kinase inhibitor Y27632 for 3 days (A) or 11 days (B). (C, D) The graph of the length of cilia labeled Ac-tubulin in A and B. (E) Scanning electron microscopy (SEM) images in undifferentiated cells treated with Y27632 for 11 days. Western blotting analysis of Ac-tubulin and γ-tubulin in undifferentiated cells treated with Y27632 for (F) 3 days or (G) 11 days. The ratio is normalized to γ-tubulin. Scale bars = 10 μm (A, B), 2 μm (E). Abbreviation: US/VOT-E36, University of Sheffield/ventral otocyst-epithelial cell line clone 36. **p<0.01 versus control.