Figure 4.

Quantitative analysis of the function of osteoclast proton pumps upon drug response. (a) Intravital two-photon fluorescence imaging of the function of osteoclast proton pumps treated with bafilomycin A1 (0.5 mg/kg). (b) Changes in fluorescence intensity normalized to the initial intensity after treatment with bafilomycin A1. (c) Intravital two-photon fluorescence imaging of the function of osteoclast proton pumps treated with the vehicle. (d) Changes in the fluorescence intensity after treatment with the vehicle. Excitated at 860 nm. Scale bars: 30 μm. Error bars represent averages ± SD (N = 13 collected from 3 independent experiments, respectively). Green, GFP-fused proton pumps; red, Red-pHocas; blue, bone tissue.