Table 1.
Nucleotide sequences and sizes of PCR products of antibiotic resistance genes and enterotoxins (A–E)
| Primer* | Oligonucleotide sequence (5’-3’)** | Gene | size of amplified product (bp) | Control strain | References |
|---|---|---|---|---|---|
| MEC-1 | AAAATCGATGGTAAAGGTTGGC | S. aureus | |||
| MEC-2 | AGTTCTGGCACTACCGGATTTGC | mecA | 533 | ATCC43300 | (17) |
| BLAZ-1 | ACTTCAACACCTGCTGCTTTC | blaZ | 240 | S. aureus | (7) |
| BLAZ-2 | TAGGTTCAGATTGGCCCTTAG | ATCCBAA-2312 | |||
| TETM-1 | GAACTCGAACAAGAGGAAAGC | S. pyogenes | |||
| TETM-2 | ATGGAAGCCCAGAAAGG T | tetM | 406 | 7020 | |
| TETK-1 | TCGATAGGAACAGCAGTA | E. faecium | |||
| TETK-2 | CAGCAGATCCTACTCCTT | tetK | 155 | ET18 | (13) |
| TETL-1 | TGGTGGAATGATAGCCCATT | ||||
| TETL-1 | CAGGAATGACAGCACGCTAA | tetL | 229 | ||
| TETO-1 | AACTTAGGCATTCTGGCTCAC | E. faecalis | |||
| TETO-2 | TCCCACTGTTCCATATCGTCA | tetO | 515 | JH2-2TET | |
| ERMA-1 | CCCGAAAAATACGCAAAATTTCAT | S. haemolyticus | |||
| ERMA-2 | CCCTGTTTACCCATTTATAAACG | ermA | 590 | 955 | |
| ERMB-1 | TGGTATTCCAAATGCGTAATG | E. faecium | (13, 14) | ||
| ERMB-2 | CTGTGGTATGGCGGGTAAGT | ermB | 745 | PE1 | |
| MSRA/B-1 | GCAAATGGTGTAGGTAAGACAACT | S. aureus | |||
| MSRA/B-2 | ATCATGTGATGTAAACAAAAT | msrA/B | 399 | RN4220 | (26) |
| ACC-1 | CAGGAATTTATCGAAAATGGTAGAAAAG | acc(6’)-le- | E. faecium | ||
| ACC-2 | CACAATCGACTAAAGAGTACCAATC | aph(2”)-la | 348 | M48 | (27, 28) |
| CFR-1 | TGAAGTATAAAGCAGGTTGGGAGTCA | ||||
| CFR-2 | ACCATATAATTGACCACAAGCAGC | cfr | 746 | (11) | |
| ESA1 | ACGATCAATTTTTACAGC | S. aureus | |||
| ESA2 | TGCATGTTTTCAGAGTTAATC | sea | 144 | FRI913 | (1) |
| ESB1 | GAATGATATTAATTCGCATC | ATCC13566 | |||
| ESB2 | TCTTTGTCGTAAGATAAACTTC | seb | 416 | (5) | |
| ESC1 | GACATAAAAGCTAGGAATTT | S. aureus | |||
| ESC2 | AAATCGGATTAACATTATCCA | sec | 257 | FRI913 | (1) |
| ESD1 | TTACTAGTTTGGTAATATCTCCTT | S. aureus | |||
| ESD2 | CCACCATAACAATTAATGC | sed | 334 | FRI151m | (3) |
| ESE1 | ATAGATAAAGTTAAAACAAGCAA | S. aureus | |||
| ESE2 | TAACTTACCGTGGACCC | see | 170 | FRI913 | (3) |
* The sets of primers were synthesised by Genomed S.A., Poland.
** The concentration of primers was 0.04 μmol