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. Author manuscript; available in PMC: 2019 Jun 28.
Published in final edited form as: Science. 2019 Mar 22;363(6433):1314–1319. doi: 10.1126/science.aav2914

Figure 6: Functional evaluation of graft-derived sperm.

Figure 6:

Sperm were derived from a cryopreserved graft retrieved from the left scrotum of13–008 that was recovered nine months after grafting. Fresh graft-derived sperm were used for the May 2017ICSI trial. The remaining sperm were cryopreserved and used for the October 2017 and November 2017 trials (See Table S2). Graft-derived sperm were used to fertilize Rhesus oocytes by ICSI (A). The resulting embryos attained 2-cell stage by day 1 (B); 8-cell stage by day 2 (C); morula stage by day 6 (D); blastocyst stage by day 10 (E); and hatching blastocyst by day 11 in culture (F). Blastocyst embryos were transferred to recipient females and a pregnancy was confirmed by ultrasound on December 12, 2017. Normal fetal development was confirmed by ultrasound on January 15, 2018 (G) and a graft-derived baby (Grady) was born by c-section on April 16, 2018 (H, image from two-week check-up).