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. 2019 Jun 21;10:705. doi: 10.3389/fphar.2019.00705

Table 1.

Protocol of CVS.

Day Stress type and schedule
1 10:00 AM, restraint stress, 60 min; 7:00 PM, humid sawdust, overnight
2 3:00 PM, cold (4°C) isolation, 60 min; 7:00 PM, lights on, overnight
3 12:00 AM, lights off, 180 min; 3:00 PM, swim stress, 4 min
4 7:30 AM, humid sawdust, all day; 7:00 PM, food/water deprivation, overnight
5 1:00 PM, swim stress, 3 min; 7:00 PM, isolation housing, overnight
6 2:00 PM cold (4°C) isolation, 15 min; 3:00 PM, lights off, 120 min
7 7:00 PM, humid sawdust and lights on, overnight
8 7:00 PM, isolation and food/water deprivation, overnight
9 4:00 PM, restraint stress, 60 min; 7:00 PM, lights on, overnight
10 8:00 AM, swim stress, 4 min; 10:00 h, restraint stress, 60 min