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. 2019 Jun 21;10:1211. doi: 10.3389/fimmu.2019.01211

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Copyright © 2019 Costa-Rocha, Campi-Azevedo, Peruhype-Magalhães, Coelho-dos-Reis, Fradico, Souza-Lopes, Reis, Freire, Costa-Pereira, Mambrini, Maia, Lima, Noronha, Xavier, Camacho, Albuquerque, Farias, Castro, Homma, Romano, Domingues, Martins, Teixeira-Carvalho and Martins-Filho.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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YF-specific phenotypic memory biomarkers 8-years after 17DD-YF primary vaccination with different doses. The YF-specific phenotypic memory was measured based on cell-surface marker expression upon in vitro 17DD-YF antigen recall as described by Costa-Pereira et al. (22). Flow cytometric phenotypic analysis based on the expression of CD45RO and CD27 was used to define memory CD4⁺ and CD8⁺ T-cell subsets: Naïve/NCD4;NCD8—(CD27⁺CD45RO⁻); early Effector/eEfCD4;eEfCD8—(CD27⁻CD45RO⁻); Central Memory/CMCD4;CMCD8—(CD27⁺CD45RO⁺) and Effector Memory/EMCD4;EMCD8—(CD27⁻CD45RO⁺). The expression of IgD and CD27 was employed to define memory B-cell subsets: Naïve/NCD19—(CD27⁻IgD⁺); Non-classical Memory/nCMCD19—(CD27⁺IgD⁺) and Classical Memory/CMCD19—(CD27⁺IgD⁻). The eligible vaccinees (n = 98) were categorized into six subgroups, according to the dose of 17DD-YF vaccine administered in 2009: 27,476IU, considered the reference dose (, n = 16); 10,447IU (, n = 17); 3,013IU (, n = 19); 587IU (, n = 17); 158IU (, n = 18), and 31IU (, n = 11). A group of non-vaccinated adult male army conscripts, referred as NV(day0), was included as a control (□, n = 46). The results are expressed mean values and standard error of 17DD-YF Ag/CC Index as described in Material and Methods. Comparative analysis for each biomarker amongst groups were assessed by ANOVA adjusted to multiple comparisons and significant differences at p < 0.05 highlighted by asterisk (^*) as compared to the mean values observed for NV(day0). The gray zone represents the mean values observed for non-vaccinated controls.

Inline graphic — YF-specific phenotypic memory biomarkers 8-years after 17DD-YF primary vaccination with different doses. The YF-specific phenotypic memory was measured based on cell-surface marker expression upon in vitro 17DD-YF antigen recall as described by Costa-Pereira et al. (22). Flow cytometric phenotypic analysis based on the expression of CD45RO and CD27 was used to define memory CD4⁺ and CD8⁺ T-cell subsets: Naïve/NCD4;NCD8—(CD27⁺CD45RO⁻); early Effector/eEfCD4;eEfCD8—(CD27⁻CD45RO⁻); Central Memory/CMCD4;CMCD8—(CD27⁺CD45RO⁺) and Effector Memory/EMCD4;EMCD8—(CD27⁻CD45RO⁺). The expression of IgD and CD27 was employed to define memory B-cell subsets: Naïve/NCD19—(CD27⁻IgD⁺); Non-classical Memory/nCMCD19—(CD27⁺IgD⁺) and Classical Memory/CMCD19—(CD27⁺IgD⁻). The eligible vaccinees (n = 98) were categorized into six subgroups, according to the dose of 17DD-YF vaccine administered in 2009: 27,476IU, considered the reference dose (, n = 16); 10,447IU (, n = 17); 3,013IU (, n = 19); 587IU (, n = 17); 158IU (, n = 18), and 31IU (, n = 11). A group of non-vaccinated adult male army conscripts, referred as NV(day0), was included as a control (□, n = 46). The results are expressed mean values and standard error of 17DD-YF Ag/CC Index as described in Material and Methods. Comparative analysis for each biomarker amongst groups were assessed by ANOVA adjusted to multiple comparisons and significant differences at p < 0.05 highlighted by asterisk (^*) as compared to the mean values observed for NV(day0). The gray zone represents the mean values observed for non-vaccinated controls.