Table 4.
Rf, Linear regression data for the calibration curve of compound 1, compound 2 and compound 3 (n = 6).
| Parameters | Compound 1 | Compound 2 | Compound 3 |
|---|---|---|---|
| Linearity range (ng/spot) | 200–1400 | 200–1400 | 200–1400 |
| Regression equation | Y = 7.517X + 518.11 | Y = 7.593X + 2193.06 | Y = 4.822X + 221.71 |
| Correlation (r2) coefficient | 0.9964 ± 0.001 | 0.9942 ± 0.002 | 0.9972 ± 0.001 |
| Slope ± SD | 7.517 ± 0.016 | 7.593 ± 0.035 | 4.822 ± 0.048 |
| Intercept ± SD | 518.11 ± 9.18 | 2193.06 ± 45.91 | 221.71 ± 16.15 |
| Standard error of slope | 0.006 | 0.014 | 0.019 |
| Standard error of intercept | 3.74 | 18.73 | 6.592 |
| Rf | 0.66 ± 0.002 | 0.60 ± 0.003 | 0.46 ± 0.003 |
| LOD (ng) | 7.42 | 15.51 | 33.21 |
| LOQ (ng) | 22.43 | 47.01 | 100.66 |