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. 2019 Jun 27;19:282. doi: 10.1186/s12870-019-1887-7

Fig. 2.

Fig. 2

Transgenerational inheritance of altered expression states of 14 genes in a single Hg-treated S0 rice plant. The mock-treated plant serves as a control, and the S0 parental line is the reference for changes in the gene expression in response to Hg-treatment. RNA was isolated from eight S1 individual progeny derived from the S0 parent. The results were highly reproducible among the three independent RNA batches, and hence, only one was presented. Gene names are listed to the left and amplification cycles are labeled to the right of the gel. Relative band intensities were used to calculate the percent progeny falling in either of the three gene expression categories: i) inheritance of Hg-treated S0 pattern, ii) reversion to the mock pattern, and iii) a differential expression pattern (predominately up-regulated expression compared to the S0 progenitor). The rice Actin gene (Genbank accession # X79378) was used as a control for normalization of RNA input. Lack of genomic DNA was validated by the Actin gene on the template without RT