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. 2019 Jun 27;19:282. doi: 10.1186/s12870-019-1887-7

Fig. 3.

Fig. 3

Transgenerational inheritance of altered expression states of seven genes in a single S1 rice plant. As evidence of inheritance of the expression states, the S0 and S1 plants are used as a reference as well as the mock control (no metal treatment). A total of 14 S2 individuals were examined to determine the expression of the Tos17A, Tos17B, SNF-FZ14 and five OsHMA transporters in the second generation. Gene names are listed to the left and amplification cycles are labeled to the right of the gel. Relative band intensities were used to calculate the percent progeny falling in either of the four gene expression categories: i) inheritance of the S1 expression state, ii) reversion to the S0 expression state, iii) reversion to the mock expression state, and iv) a novel expression pattern. The rice Actin gene (Genbank accession # X79378) was used as a control for normalization of RNA input. Lack of genomic DNA was validated by the Actin gene on the template without RT