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. 2019 Jun 27;19:282. doi: 10.1186/s12870-019-1887-7

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Determination of the Tos17 copy number using gel-blot analysis in a Hg-treated S₀ plant and its two successive offspring S₁ (1, 3, and 7) and S₂ (3, 5, and 11). The results showed that Tos17 stayed inactive, which is evident from the consistent copy number maintained in individuals of the S₀, S₁, and S₂ generations observed using a single LTR retrotransposon Tos17 specific probe (cf. Additional file 1: Table S1)