Table 2.
Transgenerational alteration and inheritance of gene expression patterns in 8 randomly chosen S1 plants derived from a Hg2+(50 μm . L−1)-treated S0 individual
| Gene name | Alteration of gene expression pattern in the S0 plant and its S1 progenies | Type and Freq. (%) of patterna | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| S0-Hg2+ | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | Inherit. of S0 pat. | Rev. to mock Pat. | New pat. (Trans. memory/others) | |
| Tos17A | U | i | +U | i | +U | i | i | +U | i | 62.5 | 0.0 | 37.5 (37.5/0.0) |
| Tos17B | U | i | +U | +U | +U | +U | +U | +U | +U | 12.5 | 0.0 | 87.5 (87.5/0.0) |
| Osr42 | U | +U | +U | +U | +U | +U | +U | +U | +U | 0.0 | 0.0 | 100.0 (100.0/0.0) |
| Homeobox gene | U | i | i | i | i | i | r | i | r | 75.0 | 25.0 | 0.0 (0.0/0.0) |
| HSP70 | U | r | i | i | i | i | i | i | i | 87.5 | 12.5 | 0.0 (0.0/0.0) |
| YF25 | D | i | i | r | r | r | -r | -r | -r | 25.0 | 37.5 | 37.5 (0.0/37.5) |
| SNF-FZ14 | U | i | i | +U | +U | +U | +U | +U | +U | 25.0 | 0.0 | 75.0 (75.0/0.0) |
| OsHMA1 | U | +U | +U | +U | +U | +U | +U | +U | +U | 0.0 | 0.0 | 100.0 (100.0/0.0) |
| OsHMA2 | U | i | D | D | i | D | i | D | i | 50.0 | 0.0 | 50.0 (0.0/50.0) |
| OsHMA4 | U | r | r | i | i | i | r | r | r | 37.5 | 0.0 | 62.5 (0.0/62.5) |
| OsHMA5 | U | i | i | r | +U | i | +U | r | i | 50.0 | 25.0 | 25.0 (25.0/0.0) |
| OsHMA6 | U | i | i | +U | +U | +U | +U | +U | +U | 25.0 | 0.0 | 75.0 (75.0/0.0) |
| OsHMA7 | U | i | +U | +U | +U | +U | +U | +U | +U | 12.5 | 0.0 | 87.5 (87.5/0.0) |
| OsHMA8 | U | i | i | i | i | +U | +U | +U | i | 62.5 | 0.0 | 37.5 (37.5/0.0) |
| OsHMA9 | U | i | i | i | i | i | i | i | i | 100.0 | 0.0 | 0.0 (0.0/0.0) |
| Average Freq. (%)a | 41.7 | 6.7 | 41.7/10.0 | |||||||||
Explanation of symbols: U = up-regulated gene expression in the S0 plant;
+U denotes further up-regulated gene expression in the S1 progeny plant;
D denotes down-regulated gene expression in the S1 progeny plants compared to the mock control
i denotes inheritance of S0 expression pattern in the S1 progeny;
r denotes reversal to Mock control expression pattern in the S1 progeny;
Note: a The average frequency of the specified pattern in S1 progeny plants. For calculations the two copies of Tos17 were treated separately,
aRelative band intensities were used to calculate the percent progeny following in either of the three gene expression categories: i) inheritance of Hg- treated S0 pattern, ii) reversion to the mock pattern, and iii) a differential expression pattern (cf. Fig. 2)