. 2019 Jun 27;18:218. doi: 10.1186/s12936-019-2833-8

Table 1.

Primer sequence of Pfmdr1 gene flanking at codon 86 and 1246

Primer	Sequence (5′ → 3′)	Direction	PCR product (bp)	Cycling condition
Pfmdr1 86 and 184	AGAGAAAAAAGATGGTAACCTCAG	Forward	590	95 °C for 3 min, 34 cycles of 95 °C for 45 s, 60.2 °C for 45 s, and 72 °C for 5 min
Pfmdr1 86 and 184	ACCACAAACATAAATTAACGG	Reverse	590
Pfmdr1 1246	GTGGAAAATCAACTTTTATGA	Forward	499	94 °C for 5 min, 35 cycles of 94 °C for 1 min, 53 °C for 1 min, 72 °C for 1 min
	TTAGGTTCTCTTAATAATGCT	Reverse	499
	GACTTGAAAAATGATCACATT	Forward nested	409	94 °C for 5 min, 35 cycles of 94 °C for 1 min, 50 °C for 1 min, 72 °C for 1 min
	GTCCACCTGATAAGCTTTT	Reverse nested	409