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. 2019 Jun 27;10:193. doi: 10.1186/s13287-019-1303-0

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — GFP-labeled PC-iPS cells contribute to chimeric formation in pig embryos in vitro and in vivo. (A, a) Representative fluorescence images of pig embryo injected with GFP-labeled PC-iPS cells, scale bar 10 μm. (A, b) GFP-labeled PC-iPS contribute to the pig ICM, scale bar 50 μm. (A, c) GFP-labeled PC-iPS cells contribute to the pig TE, scale bar 50 μm. (B) Nested PCR analysis of GFP using post-implantation pig fetal embryonic and extraembryonic tissues (the GFP-labeled PC-iPS cells’ DNA and PB513B-1 plasmid were used as GFP sequence positive controls; double distilled water (ddH₂O) and mouse DNA were used as negative controls)