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. 2019 Jun 27;21:76. doi: 10.1186/s13058-019-1157-5

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Expression of genes in AH and regulation by SFRP1. Genes associated with AH were identified by AGNES and PAM were examined for regulation by SFRP1. a The relative expression of the genes in AH are shown. Green indicates genes with decreased expression in AH relative to the HNB tissues and red indicates those with increased expression. b The effect of SFPR1 knockdown on gene expression was analyzed in a cell line derived from normal breast epithelium and immortalized with telomerase (76NTERT cells). Relative levels of transcripts were determined by RT-qPCR in TERT-siSFRP1 cells and the TERT-pSUPER vector control cells. c Similarly, mRNA levels of the genes was compared by RT-qPCR in mouse mammary glands derived from Sfrp1^−/− and control Sfrp1^+/+ mice. d Breast cancer cells overexpressing SFRP1 (MCF7-SFRP1) and control cells (MCF7-pCDNA) were also compared for relative levels of gene expression by RT-qPCR. The level of SFRP1 mRNA was normalized to the amplification of ACTB mRNA, which was performed in parallel wells for each cell line or tissue. Bars represent mean ± SEM SFRP1/ACTB and are expressed as relative expression of control TERT-pSUPER (b), control mice (c), and MCF7-pCDNA (d). Genes where loss of SFRP1 results in decreased expression are shown in green, those increased are shown in red, and those which are unchanged are shown in white *p < 0.05, **p < 0.01, and ***p < 0.001 (significantly different from control using Student’s t test)