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. 2019 Jun 27;20:115. doi: 10.1186/s12881-019-0819-6

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Evaluation of PCR-ALDA compared to Sanger sequencing and real-time PCR. Venn diagram and bar graph illustrating the distribution of Jak2 V617F and CALR mutations among 105 MPN patients. Screening for Jak2 V617F was performed by ARMS-PCR and detection of CALR mutants by PCR-ALDA, real-time PCR (rt-PCR) and by direct Sanger sequencing (SQ)