Table 3.
Gene | Primer sequences (5′ → 3′) | Amplicon, bpa | Accession No. |
---|---|---|---|
SOD1 b | F: AGATGGCAGTGGGAAATGAG | 110 | NM_205064.1 |
R: ACTCAAGACAGCAGAGTAGTAATG | |||
CAT | F: GAGGAACCCTCAGACTCATTTG | 117 | NM_001031215.2 |
R: CCATCAGGAATACCACGATCAC | |||
PEPT1 | F: CCCCTGAGGAGGATCACTGTT | 66 | KF366603.1 |
R: CAAAAGAGCAGCAGCAACGA | |||
y + LAT-1 | F: TGTTGGAGCCAGAGAAGGA | 118 | XM_418326.6 |
R: CACAAGGAGATAAAGCAAAGTC | |||
B 0 AT1 | F: TCTATTGAAGATTCGGGCAC | 153 | XM_419056.6 |
R: AATGGTAAGCACAAGGTATGG | |||
CAT - 1 | F: CGTGGCATCTCTGCTCATC | 134 | NM_001145490.1 |
R: CTCCATCCCAACCTACATACTTA | |||
TLR2 | F: ACTGCCTGCAACGGTCAT | 75 | NM_204278.1 |
R: CATCAGCTTCATTGTTGGTTTCTGT | |||
TLR5 | F: ACACGGCAATAGTAGCAACACATAT | 91 | XM_025148815.1 |
R: ACACCTGGAACTTGGAAAAGAACAT | |||
IL1B | F: GTCAACATCGCCACCTACAA | 90 | XM_015297469.1 |
R: CGGTACATACGAGATGGAAACC | |||
IL2 | F: CCTCAAGAGTCTTACGGGTCTA | 104 | GU119890.1 |
R: AGTTGGTCAGTTCATGGAGAAA | |||
IFNG | F: TGAGGTGATGTTTACCGAGTTT | 94 | NM_205149.1 |
R: GCTTAGAGCTGAGCAGGTATG | |||
LITAF | F: GAGCGTTGACTTGGCTGTC | 64 | NM_204267.1 |
R: AAGCAACAACCAGCTATGCAC | |||
CLDN1 | F: ACTCCTGGGTCTGGTTGGT | 100 | AY750897.1 |
R: GACAGCCATCCGCATCTTCT | |||
OCLN | F: ACGGCAGCACCTACCTCAA | 123 | D21837.1 |
R: GGGCGAAGAAGCAGATGAG | |||
iNOS | F: TCCTGAGTTCTGTGCCTTTG | 92 | U46504.1 |
R: GTTCATCTCCTTCACCCACTG | |||
ACTB | F: GCCAACAGAGAGAAGATGAC | 130 | L08165.1 |
R: CACCAGAGTCCATCACAATAC |
abp Base pairs
bSOD1 Superoxide dismutase 1 gene, CAT Catalase gene, PEPT1 Peptide transporter 1 gene, y+LAT-1 Neutral and cationic amino acid transporter 1 gene, B0AT1 System B0 neutral amino acid transporter 1 gene, CAT–1 Cationic amino acid transporter 1 gene, TLR2 Toll-like receptor 2 gene, TLR5 Toll-like receptor 5 gene, IL1B Interleukin 1 beta gene, IL2 Interleukin 2 gene, IFNG Interferon gamma gene, LITAF Lipopolysaccharide-induced tumor necrosis factor-alpha factor gene, CLDN1 Claudin-1 gene, OCLN Occludin gene, iNOS Inducible nitric oxide synthase gene, F Forward, R Reverse