Table 5.
Carbonylated protein, CP, total antioxidant capacity, TAC, and antioxidant enzyme activities in the jejunum of broilers 144 h post-inoculation with Eimeria spp
CPd | TACe | Catalasef | SODg | ||||||
---|---|---|---|---|---|---|---|---|---|
Mean | SE | Mean | SE | Mean | SE | Mean | SE | ||
UCh | NSj | 1.05bc | 0.11 | 64.36c | 3.52 | 28.27a | 2.06 | 4.49ab | 0.09 |
dl-Metk | 1.04bc | 0.17 | 81.89a | 2.15 | 21.47bc | 1.42 | 3.82c | 0.04 | |
dl-MMetl | 1.27ab | 0.16 | 71.39bc | 1.69 | 27.78a | 1.28 | 3.69c | 0.13 | |
ECi | NS | 1.43a | 0.10 | 65.27c | 2.10 | 19.62c | 1.25 | 4.72a | 0.05 |
dl-Met | 0.85c | 0.15 | 67.45c | 3.06 | 24.77ab | 1.94 | 4.32b | 0.11 | |
dl-MMet | 0.90bc | 0.07 | 78.32ab | 2.52 | 22.38bc | 0.49 | 3.59c | 0.14 | |
P-value | |||||||||
Challenge × Diet | 0.0200 | 0.0008 | 0.0012 | 0.0214 |
a,b,cMeans in the same column followed by different letters differ significantly by the Tukey test, P < 0.05. Results are presented as mean ± standard error, SE. Each bird was considered an experimental unit, n = 6
dExpressed as nmol of carbonyl groups/mg of protein
eTAC = total antioxidant capacity, expressed as percentage, %
fCatalase activity, expressed as the amount of hydrogen peroxide, H2O2 consumed per minute per milligram of protein
gSOD = superoxide dismutase activity, expressed as SOD unit/mg of protein
hUC = unchallenged broilers
iEC = Eimeria-challenged broilers
jNS = non-supplemented diet
kdl-Met = diet supplemented with free methionine (dl-methionine 99%)
ldl-MMet = diet supplemented with methionine dipeptide (dl-methionyl-dl-methionine 95%)