Table 2.
Primer | Sequence (5ʹ→ 3ʹ) a |
---|---|
f-5ʹompA_SphI | ATCTGCATGCTTTCGAGGAAGAACCGATGGTTGC |
r-5ʹompA_SalI | ATACGTCGACAATATAGCGGACTGCAATCC |
f-3ʹompA_BamHI | ACTTGGATCCTTCTGAATCGTGTGGTATTGG |
r-3ʹompA_SacI | ACTAGAGCTCATCTGTAGAGAAGAAACGGG |
SPBTOmpA_fwd | CATGTTGCTGGCTTTTGCCGGCGTTGCGTCTGTCGCTTCTG CGCAGCAAACCGTGACTGTAACTGAATACGAGGTTATTCATATGTGACG |
SPBTOmpA_rev | AATTCGTCACATATGAATAACCTCGTATTCAGTTACAGTCACGG TTTGCTGCGCAGAAGCGACAGACGCAACGCCGGCAAAAGCCAGCAA |
OmpAST_fwd | TGACCATATGGCTCCGAAAGATAACACC |
OmpAST_rev | GTCAGAATTCTTAAGCCTGCGGCTGAGTTA |
SseB_fwd | TGACCATATGTCTTCAGGAAACATCTT |
SseB_rev | TGACGAATTCATGAGTACGTTTTCTGCG |
XhoI_STOmpA_rev | ATATCTCGAGGAAACTTAAGCCTGCGG |
XhoI_SseB_rev | ATATCTCGAGATGAGTACGTTTTCTGCG |