Figure 4.

Supernatant paracrine effect of P. gingivalis and F. nucleatum monospecies biofilms after exposure to blue light. (a) – P. gingivalis and F. nucleatum biofilms exposed to blue light (test group) or non-exposed (control group) after a supernatant exchange of the two biofilms. (b) – F. nucleatum biofilm, non-exposed and exposed to blue light, after the addition of supernatants of P. gingivalis biofilm (exposed or non-exposed) and without supernatant (control). (c) – F. nucleatum biofilm non-exposed to light, with the addition of supernatant of exposed P. gingivalis biofilm, alone and with ROS scavengers (vitamin C or catalase) or protease inhibitor. The results are expressed as the mean ± SD. * and ^# = significant difference from the other groups.