Figure 2.

Cisplatin is a potent inhibitor of the Prp8 intein in vitro. (A) The Prp8 intein splicing assay based on split luciferase. RLuc (2 nM) or RLuc-Prp8 (2 nM) was used with DMF or cisplatin (40 µM) with/without 100 µM TCEP. Reactions were incubated at room temperature for 18 h, followed by luminescence detection. N=8. ***, p<.001. (B) Dose-response fitting of inhibition of splicing of the RLuc-Prp8 by cisplatin. RLuc-Prp8 (2 nM) was used. Cisplatin was in twofold serial dilutions with concentrations ranging from 100 µM (30 µg/ml) to 0.78 µM (0.23 µg/ml). N=3.