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. 2019 Jun 21;9:526. doi: 10.3389/fonc.2019.00526

Figure 5.

Figure 5

SMAD4 regulation by miRs-205, −146a, and −1260b induces proliferation and migration in Hec1a cells. (A,B) Hec1a cells proliferation was measured at 72 h. Cells were transiently transfected with negative control #1 (C) and miRs-205, −146a, −1260b in A or anti-miRs-205, −146a, and −1260b (a-miRs) in B. (C) Transwell migration assay shows that overexpression of miRs-205, −146a, −1260b, and siSMAD4 enhance cell migration ability of Hec1a cells. (D) Representative photographs of the Transwell migration assay is shown. All data are presented as mean ± SD, *p < 0.05. (E,F) Effects of siSMAD4 expression in Hec1a cells. (E) RT-PCR (upper panel) and western blot analysis (lower panel) are shown. LC (loading control, β-actin). PCR bars depict SMAD4 expression in control (ctrl) and si transfected cells normalized to β-actin. (F) Measure of Hec1a cells proliferation in siSMAD4 and SiCTRL transfected cells is reported. Data represent the mean (from three independent experiments) ±SD. *P < 0.05.