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. Author manuscript; available in PMC: 2019 Jun 28.
Published in final edited form as: Sci Signal. 2019 Jan 15;12(564):eaat8715. doi: 10.1126/scisignal.aat8715

Fig. 4. Dwell time accumulation does not set the activation threshold.

Fig. 4

(A and B) The number of pMHC-TCR binding events (A) and their total dwell time (B) that T cells accumulated at the time of initial NFAT activation. Cells were exposed to distinct input densities with different NFAT activation probabilities: 32% at threshold T102S pMHC density (blue), 41% at threshold MCC density (red), and 5% at low T102S density (dark blue). Scatterplot of single-cell values after correction for events that are not directly observed with population means (black) ± SD (gray) and estimated population distributions (right) from 11 cells from five experiments (Threshold MCC), 15 cells from seven experiments (Threshold T102S), or 7 cells from three experiments (Low T102S).