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. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: Transl Stroke Res. 2018 Feb 24;9(5):506–514. doi: 10.1007/s12975-018-0611-5

Fig. 4.

Fig. 4

Inhibition of Rac1 reduced axonal length and numbers after OGD. Microfluidic chamber was used to separate axons from neuronal soma. At 6 days in vitro, cortical neurons co-cultured with 30 μM NSC23766 for 96 h after challenged by OGD for 2 h, axonal length (a) and number (b) were analyzed by immunocytochemistry NFL staining. N = 4, *p < 0.05. Scale bars: 50 μm. c NSC incubation had no effect on cortical neuron’s cell viability. N =4, p > 0.05. Data were presented as mean ± SEM