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. 2019 Jun 20;10:1348. doi: 10.3389/fmicb.2019.01348

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Copyright © 2019 Maiden, Zachos and Waters.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Melittin in combination with tobramycin is additive and synergistic. PAO1 P. aeruginosa biofilms were treated for 6-h with checkerboard dilutions of melittin combined with tobramycin. Number of viable cells within the biofilms were quantified by BacTiter-Glo^TM. The assay was performed twice in triplicate. The results represent means.