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. 2019 Jun 12;10:1349. doi: 10.3389/fimmu.2019.01349

Figure 2.

Figure 2

Antigen internalization and processing in human DC after delivery by PS liposome. (A) Immature and mature DC cells were exposed to dialyzed PS liposomes loaded with AF647-BSA to a ratio of 5:1 for 1 h. Analysis was performed by flow cytometry. (B) Immature DC cells were stained with the nucleic acid stain Hoechst and the acidophilic dye Lysotraker Red and stimulated with PS liposomes loaded withAF647-BSA at the ratio 5:1 for 90 min. A representative image from over many taken by confocal microscopy, is shown. Sample analysis indicated majority of cells positively stained with ~23% strongly positive for both stains. (C) Immature DC cells were exposed to PS liposomes loaded with DQ-OVA or to empty liposomes at a ratio of 5:1 for 1 h. Red and green fluorescence was evaluated by flow cytometry. A representative experiment with cells from one, out of three, healthy donors is shown.