Figure 3.

Reduced Mtb infection in Lipo-AE immunized mice. Four weeks after the final immunization, mice were challenged with aerosolised Mtb and then 4 weeks later culled and organs harvested for bacterial enumeration. Each point corresponds to log CFU value for the individual animals (n = 7). (A) Schematic depicting immunization and Mtb infection schedule. (B) CFU in the lungs and spleens. The horizontal bars represent the mean for each group ± SEM. Log transformed data were analyzed using a 1-way ANOVA and a Dunnett's multiple comparison test comparing all groups to the BCG control or comparing Lipo-AE with empty liposomes (Lipo); *P ≤ 0.05, **P ≤ 0.01, ****P ≤ 0.0001.