Figure 4.

Mucosal antibody responses and lung Trm induced by Lipo-AE. (A) Levels of anti-Ag85B specific BAL IgG and IgA determined by antigen-specific ELISA. Each bar corresponds to the mean of triplicate samples from 3 animals from each group ± SEM. The set of bars represent 3-fold serial dilutions from the starting neat sample. Average end point titres are indicated above the bars. (B) Analysis of the lung resident memory T cell populations. Except for the PBS group, animals were vaccinated with BCG sc and then with Lipo-AE or empty liposomes, both containing PolyIC, as described in Methods. Cells isolated from the lungs of immunized animals were stained and analyzed by flow cytometry to determine the Trm populations. The gating strategy used was cells -> single cells -> live cells -> CD3+ -> CD4+/CD8+ -> CD44 ^high/CD62L^low and the CD69/CD103 double positive Trm presented. Data originated from n = 3 pooled animals and a representative plot is shown.