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. 2019 Jun 12;10:1349. doi: 10.3389/fimmu.2019.01349

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Copyright © 2019 Diogo, Hart, Copland, Kim, Tran, Poerio, Singh, Paul, Fraziano and Reljic.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of the lung resident memory T cell populations. Except for the PBS group, animals were vaccinated with BCG sc and then received one s.c. and one i.n. administration of Lipo-AE or empty PS/PS liposomes with PolyIC. Cells isolated from the lungs of immunized animals were stained and analyzed by flow cytometry to determine the Trm populations. The gating strategy used was cells -> single cells -> live cells -> CD3+ -> CD4+/CD8+ -> CD44 ^high / CD62L^low and the CD69/CD103 double positive Trm presented. Data originated from n = 3 pooled animals and a representative plot is shown.