Figure 1.

Expression of the selected genes as candidate biomarkers of vitD3-tolDC. (A) Expression of CA2, GZMB and SNORD30 in healthy donors by microarray (n = 5) and quantitative PCR (qPCR; n = 20, except CA2, in which n = 24) in immature dendritic cells (iDC), mature DC (mDC) and vitD3-tolDC. (B) Expression of CAMP, CLEC5A, CYP24A1, MAP7 and MUCL1 in healthy donors both by microarray (n = 5) and qPCR analysis (n = 24, except CYP24A1, in which n = 10) in iDC, mDC and vitD3-tolDC, and in multiple sclerosis (MS) patients by qPCR only (n = 10). (C) Expression of MAP7 and MUCL1 in vitD3-tolDC (n = 24) and in IL10-tolDC (n = 5) by qPCR. Data presented as the mean difference of expression (MeanDiff) or the decimal logarithm of fold change (logFC) expression for the microarray and qPCR results, respectively, in both cases normalized to mDC expression. Housekeeping genes GAPDH, TBP and CYPA were used as controls. One qPCR experiment was performed for each donor or patient, with triplicated measurements for each sample. Error bars corresponding to SEM. Dotted lines represent the logFC = 0.5 or −0.5 expression threshold. *p < 0.05. Friedman test with Dunn's correction, one-way ANOVA test with Geisser-Greenhouse correction or paired t-test.