Figure 3.

Immunocytochemistry study of MAP7 and MUCL1 protein expression in dendritic cells. (A) Relative levels of expression of microtubule-associated protein 7 (MAP7) in dendritic cells differentiated from healthy donor samples (n = 4) and MS patient samples (n = 3). (B) Relative levels of expression of mucin-like 1 (MUCL1) in dendritic cells differentiated from healthy donor samples (n = 4) and MS patient samples (n = 3). Results are calculated as percentage (%) of corrected total cell fluorescence (CTCF) in immature dendritic cells (iDC) and mature DC (mDC) compared to vitD3-tolDC. One single immunocytochemistry experiment was performed for each sample. Error bars corresponding to SEM. *p < 0.05. One-way ANOVA test with Geisser-Greenhouse correction or paired t-test. Representative pictures of the expression of (C) MAP7 and (D) MUCL1 in iDC, mDC and vitD3-tolDC from healthy donor and MS patient samples. α-tubulin staining is shown in green; either MAP7 or MUCL1 staining is shown in red; nuclei staining is shown in blue. The immunocytochemistry analysis was performed on a fluorescence microscope using a 63x objective. Immunocytochemistry primary staining was performed using mouse anti-human α-tubulin and either rabbit anti-human MAP7 or rabbit anti-human MUCL1 antibodies. Secondary stainings were performed using AlexaFluor (AF) 488 goat anti-mouse IgG and AF 594 goat anti-rabbit IgG antibodies. Nuclei staining was performed using DAPI.