Table 1.
Expression by microarray and qPCR of the selected genes in dendritic cells differentiated from healthy donors and multiple sclerosis patients.
| MICROARRAY DATA FROM HEALTHY DONORS [FROM NAVARRO-BARRIUSO ET AL. (33)] | ||||||||
|---|---|---|---|---|---|---|---|---|
| iDC vs. mDC | vitD3-tolDC vs. mDC | |||||||
| Gene | MeanDiff | p-value | MeanDiff | p-value | ||||
| CA2 | 1.044 | 0.097 | 1.680 | 0.010 | ||||
| CAMP | 0.337 | 0.279 | 1.351 | <0.001 | ||||
| CLEC5A | 0.015 | 0.975 | 1.573 | 0.003 | ||||
| CYP24A1 | −0.585 | 0.264 | 2.271 | <0.001 | ||||
| GZMB | 0.097 | 0.582 | 0.734 | <0.001 | ||||
| MAP7 | 0.075 | 0.663 | 0.880 | <0.001 | ||||
| MUCL1 | 0.084 | 0.857 | 2.132 | <0.001 | ||||
| SNORD30 | −0.040 | 0.949 | 1.616 | 0.016 | ||||
| HEALTHY DONORS | ||||||||
| iDC vs. mDC | vitD3-tolDC vs. mDC | vitD3-tolDC vs. iDC | ||||||
| Gene | logFC±SD | p-value | logFC±SD | p-value | logFC±SD | p-value | ||
| CA2 | 0.566 ± 0.391 | 0.001 | 0.858 ± 0.445 | <0.001 | 0.292 ± 0.276 | 0.042 | ||
| CAMP | 0.290 ± 0.397 | 0.042 | 0.957 ± 0.447 | <0.001 | 0.668 ± 0.433 | 0.003 | ||
| CLEC5A | −0.254 ± 0.371 | 0.063 | 0.602 ± 0.324 | 0.001 | 0.856 ± 0.346 | <0.001 | ||
| CYP24A1 | −1.680 ± 0.422 | 0.008 | 1.532 ± 0.637 | 0.004 | 3.212 ± 0.914 | 0.004 | ||
| GZMB | 0.588 ± 0.426 | 0.001 | 0.607 ± 0.554 | 0.001 | 0.019 ± 0.515 | >0.999 | ||
| IL1R1 | −0.133 ± 0.259 | 0.250 | −0.662 ± 0.353 | <0.001 | −0.528 ± 0.293 | <0.001 | ||
| MAP7 | 0.473 ± 0.353 | 0.002 | 1.015 ± 0.220 | <0.001 | 0.542 ± 0.308 | 0.007 | ||
| MUCL1 | −0.318 ± 0.453 | 0.130 | 1.511 ± 0.419 | <0.001 | 1.829 ± 0.405 | <0.001 | ||
| SNORD30 | −0.025 ± 0.204 | 0.056 | −0.001 ± 0.207 | 0.233 | 0.024 ± 0.278 | >0.999 | ||
| MULTIPLE SCLEROSIS PATIENTS | ||||||||
| iDC vs. mDC | vitD3-tolDC vs. mDC | vitD3-tolDC vs. iDC | ||||||
| Gene | logFC±SD | p-value | logFC±SD | p-value | logFC±SD | p-value | ||
| CAMP | 0.431 ± 0.342 | 0.042 | 0.441 ± 0.331 | 0.076 | 0.010 ± 0.459 | >0.999 | ||
| CLEC5A | −0.837 ± 0.539 | 0.002 | 0.111 ± 0.249 | 0.668 | 0.947 ± 0.490 | 0.002 | ||
| CYP24A1 | −0.762 ± 0.739 | 0.160 | 1.465 ± 0.908 | <0.001 | 2.227 ± 1.448 | <0.001 | ||
| IL1R1 | 0.143 ± 0.158 | 0.103 | −0.307 ± 0.179 | 0.002 | −0.450 ± 0.214 | 0.008 | ||
| MAP7 | 0.320 ± 0.296 | 0.081 | 0.506 ± 0.297 | 0.003 | 0.187 ± 0.292 | 0.174 | ||
| MUCL1 | −0.283 ± 1.209 | 0.221 | 0.790 ± 0.325 | 0.042 | 1.073 ± 1.362 | <0.001 | ||
Gene expression values from a microarray study with healthy donors (n = 5) or from qPCR analysis from healthy donors (n = 24, except for CYP24A1, in which n = 10, and GZMB and SNORD30, in which n = 20) and multiple sclerosis patients (n = 10). Data presented as either the mean difference of expression (MeanDiff) for the microarray data or as the mean decimal logarithm of fold change (logFC) ± SD for the qPCR results. Housekeeping genes GAPDH, TBP and CYPA were used as controls. One qPCR experiment was performed for each donor or patient, with triplicated measurements for each sample. Microarray p-values < 0.01 and qPCR p-values < 0.05 are highlighted in bold. iDC, immature dendritic cells; mDC, mature dendritic cells; vitD3-tolDC, vitamin D3-induced tolerogenic dendritic cells. Friedman test with Dunn's correction or one-way ANOVA test with Geisser-Greenhouse correction.