Table 2.
Expression by microarray and qPCR of the selected genes from the protein interaction network in dendritic cells differentiated from healthy donors and multiple sclerosis patients.
| MICROARRAY DATA FROM HEALTHY DONORS [FROM NAVARRO-BARRIUSO ET Al.(33)] | ||||
|---|---|---|---|---|
| vitD3-tolDC vs. mDC | vitD3-tolDC vs. iDC | |||
| Gene | MeanDiff | p-value | MeanDiff | p-value |
| DHRS9 | 1.160 | 0.007 | −0.161 | 0.685 |
| IL2RA | −1.539 | 0.040 | 2.297 | 0.003 |
| OS9 | −0.014 | 0.926 | −0.478 | 0.004 |
| SPARC | 1.347 | <0.001 | 0.506 | 0.101 |
| ST6GAL1 | 1.564 | <0.001 | 0.189 | 0.570 |
| THBS1 | −1.052 | 0.048 | 1.944 | <0.001 |
| VEGFA | 0.200 | 0.509 | 0.696 | 0.028 |
| qPCR DATA FROM HEALTHY DONORS | ||||
| vitD3-tolDC vs. mDC | vitD3-tolDC vs. iDC | |||
| Gene | logFC±SD | p-value | logFC±SD | p-value |
| DHRS9 | 0.683 ± 0.387 | 0.002 | n/a | |
| OS9 | −0.011 ± 0.172 | 0.917 | −0.265 ± 0.182 | 0.015 |
| SPARC | 1.210 ± 0.346 | 0.003 | n/a | |
| ST6GAL1 | 0.571 ± 0.223 | 0.002 | n/a | |
| THBS1 | −0.023 ± 0.285 | 0.694 | 2.508 ± 0.303 | 0.004 |
| qPCR DATA FROM MULTIPLE SCLEROSIS PATIENTS | ||||
| vitD3-tolDC vs. mDC | vitD3-tolDC vs. iDC | |||
| Gene | logFC±SD | p-value | logFC±SD | p-value |
| DHRS9 | 0.264 ± 0.358 | 0.557 | n/a | |
| OS9 | −0.036 ± 0.152 | >0.999 | −0.190 ± 0.222 | 0.076 |
| SPARC | 0.552 ± 0.194 | 0.011 | n/a | |
| ST6GAL1 | 0.403 ± 0.331 | 0.001 | n/a | |
| THBS1 | −0.027 ± 0.216 | 0.420 | 2.391 ± 0.381 | 0.002 |
Gene expression values from a microarray study with healthy donors (n = 5) or from qPCR analysis from healthy donors (n = 10) and multiple sclerosis patients (n = 10). Data presented as either the mean difference of expression (MeanDiff) for the microarray data or as the mean decimal logarithm of fold change (logFC) ± SD for the qPCR results. Housekeeping genes GAPDH, TBP and CYPA were used as controls for the qPCR experiments. One qPCR experiment was performed for each donor or patient, with triplicated measurements for each sample. Microarray p-values < 0.01 and qPCR p-values < 0.05 are highlighted in bold. iDC, immature dendritic cells; mDC, mature dendritic cells; vitD3-tolDC, vitamin D3-induced tolerogenic dendritic cells. Friedman test with Dunn's correction, one-way ANOVA test with Geisser-Greenhouse correction, Wilcoxon matched-pairs signed rank test or paired t-test.