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. 2019 May 14;92(20):e2364–e2374. doi: 10.1212/WNL.0000000000007505

Figure 5. Immunofluorescent staining of ciliary protein.

Figure 5

(A) Decrease in number of acetylated tubulin (white arrowheads) was found in the choroid plexus of Cfap43−/− mice. Green: ac-tubulin, marker of cilia. Blue: DAPI. White scale bar = 50 μm. (B) Some epithelial cells of the lateral ventricle and trachea of Cfap43−/− mice showed defect of Spef2 protein. Green: ac-tubulin, marker of cilia. Red: Spef2, a marker of the axoneme central pair. Blue: DAPI. White scale bar = 20 μm. (C) Some epithelial cells of the lateral ventricle and trachea of Cfap43−/− mice showed a defect of Rsph4a protein expression. Green: ac-tubulin, a marker of cilia. Red: Rsph4a, a marker of radial spokes. Blue: DAPI. White scale bar = 20 μm. (C) Some epithelial cells of the lateral ventricle and trachea of Cfap43−/− mice showed a defect of Rsph4a protein expression. Green: ac-tubulin, a marker of cilia. Red: Rsph4a, a marker of radial spokes. Blue: DAPI. White scale bar = 20 μm. WT = wild-type.