Fig. 1.

NOX4 Antibody validation, NOX4 expression pattern in the normal human glomerular compartment, and in renal nephropathies.

Representative Western blot analysis of T-Rex cells overexpressing (right lane) or not (left lane) the human NOX4 protein (A). Representative images of NOX4 immunohistochemical analysis of T-REX cells overexpressing (tetracycline inducible) (C) or not (B), the human NOX4 protein. D–F: Representative images of NOX4 immunohistochemical analysis of normal human kidney, diabetic and IgA nephropathies. In normal glomeruli, NOX4 expression is restricted to parietal glomerular epithelial cells (D, upper and lower panel). NOX4 expression is observed in the glomerular mesangium of diabetic nephropathy (E, upper and lower panel) and IgA nephropathy (F, upper and lower panel).