Figure 3.
Expression of IL-15 by rRABV enhances the maturation of DC in immunized mice. Balb/c mice were immunized with 106 FFU of LBNSE or LBNSE-IL15, or mock immunized with DMEM via i.m. route. Lymph nodes and blood were harvested at 3 and 6 days and stained with antibodies against DCs. (A) Representative flow cytometric plots of mature DCs (CD11c+CD86+ or CD11c+MHC II+) Percentage of mature DCs detected in lymph nodes (B and C) and blood (D and E) of mice immunized with different rRABVs. Data are representative of ≥ 2 independent experiment with duplicate samples. Asterisks indicate significant differences between the experimental groups as analyzed by unpaired t test, data is presented at the mean ± SEM. *, P < 0.005;**, P < 0.01;***, P < 0.001; (N = 3 mice per group).