Fig. 5.

On-chip parallel formation of a library of liposomes or lipoplexes. a Schematic description of lipoplex formation. An array (HSTL slide C) of aqueous sodium acetate buffer droplets containing sucrose, gelatin, fibronectin and pDNA (pCS2-GFP) was sandwiched with LSTL slide A containing a library of dried lipidoids, followed by incubation at 50 °C for 1.5 h and drying before using the slide C for the following reverse cell transfection experiment. Liposomes were produced in the same way without adding plasmid DNA. b Results of dynamic light scattering (DLS) and zeta potential analyses of lipoplexes and corresponding liposomes. Liposomes display smaller particles and higher zeta potential than corresponding lipoplexes.+/− values are standard deviations, n = 3 (number of replicates). Source data are provided as a Source Data file