Figure 3.

In vivo confirmation of the RNA sequencing results. (A) β-Galactosidase activity of different promoter-lacZ fusions in S. melonis Fr1 wild type (black bars) and the ΔecfG mutant (grey bars) under low stress conditions (TYE medium) to confirm dependency of the corresponding genes on EcfG. Values are given as mean ± SD of three independent experiments. (B) Transcript IDs, gene functions, and log₂ fold change ratios of the corresponding genes resulting from the RNA sequencing data relating to the ΔecfG vs wild type comparison under low stress conditions are indicated; the false discovery rate (fdr) is below 0.05 for all listed genes. Log₂ fold change ratios were rounded to one decimal place.