Fig. 2.

High death effector domain-containing DNA-binding protein (DEDD) expression facilitates G1/S progression in triple-negative breast cancers (TNBCs). a Western blots showing the endogenous DEDD expression across multiple TNBC cell lines. b Cell counting assay showing cell relative cell viability after DEDD knockdown in three TNBC cell lines. c Clonogenic assay showing relative colony number changes after DEDD knockdown in MDA-MB-468 cells. d, Left: Representative images showing 5-ethynyl-2′-deoxyuridine (EdU) incorporation in TNBC cells with or without short hairpin DEDD (shDEDD) expression. 4′,6-Diamidino-2-phenylindole (DAPI) staining (blue); EdU staining (orange). Right: Quantification of EdU incorporation. e Representative flow biaxial plots showing cell cycle population distribution in TNBC cells with or without shDEDD expression. f Immunoblots showing cyclin B1 and cyclin D1 expression changes with or without small interfering DEDD (siDEDD) knockdown at 0 and 9/6 h after abolishing synchronization by fetal bovine serum (FBS) in HCC38/HCC1806/MDA-MB-468 cells. All quantitative data were generated from a minimum of three replicates. P values were derived from one-way analysis of variance (ANOVA) with Dunnett’s multiple comparison test comparing different shRNAs to the PLKO.1 group. Error bars represent means ± s.e.m