Fig. 3.

Generation of LMNA^G608G pig via direct zygote injection. a Summary of generation of LMNA^G608G mutant pigs by using direct zygote injection of the BE3 system. b Representative photograph of newborn LMNA^G608G piglets. c Summary of genotypes of nine newborn piglets from targeted deep sequencing. C-to-T substitutions and indels are shown in red. d Sanger sequencing chromatograms of WT, 357-5, and 357-8 piglets. The red arrow indicates the target sites with C-to-T conversions. e The efficiencies of C-to-T and non C-to-T substitutions in all Cs in LMNA-sgRNA were detected by targeted deep sequencing. f The expression of WT and truncated LMNA was detected by RT-PCR. Truncated LMNA mRNA is translated to progerin, which can result in HGPS. g Sanger sequencing chromatograms of RT-PCR products of WT and LMNA^G608G piglets. h Western blot was used to detect the expression of lamin A/C and progerin protein in the heart, liver, spleen, lung, kidney, and ear tissues of WT and 357-8 piglets. Source data are provided as a Source Data file