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. 2019 Jun 28;10:2876. doi: 10.1038/s41467-019-10753-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Deficiency of miR-204/-211 upregulates Runx2, cartilage-catabolic enzymes and osteogenic markers. a IHC results show enhanced Runx2 expression in the synovium, articular cartilage (AC), and meniscus of knee joints of dKO mice. Red arrowheads, Runx2-positive cells. Scale bar, 50 μm. b Western blot analysis shows Runx2 accumulation in CD45⁻ BMSCs isolated from dKO mice. The numbers below the blot indicate the relative protein levels of Runx2 (normalized to β-actin) in each group compared to the control. n = 3. c IHC results of MMP13 and Adamts5 in control and dKO mice. Arrowheads, IHC-positive cells. Scale bar, 100 μm. d qRT-PCR analysis of Mmp13, Adamts5, nerve growth factor (NGF), and Runx2 expression in articular chondrocytes from control or dKO mice. Unpaired Student’s t test. n = 3. Data are shown as the mean ± s.d. e−g Alkaline phosphatase (ALP) staining (e), Alizarin red staining (f), and von Kossa staining (g) of BMSCs isolated from control or dKO mice. n = 3. h qRT-PCR analysis of Runx2, Osterix (Osx), Alp and Osteocalcin (OC) expression in CD45⁻ BMSCs from control or dKO mice. Unpaired Student’s t test. n = 3. Data are shown as the mean ± s.d. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Uncropped western blot scans are shown in Supplementary Data 1