Fig. 6.

Intraarticular injections of AAV5-miR-204 alleviates surgically induced OA phenotype. a Representative μCT images of osteoarthritic joints in 9-month-old C57BL/6 mice receiving AAV5-GFP or AAV5-miR-204 injection at the age of 3 months. Red arrowheads, osteophytes. n = 5. Scale bar, 1 mm. b Representative histology images of osteoarthritic joints in 9-month-old mice receiving AAV injection at the age of 3 months. Yellow arrowheads, articular cartilage degradation; green arrowhead, synovial hyperplasia; black arrowhead, subchondral sclerosis. n = 5. Scale bars, 200 μm. c Analysis using OARSI scoring system was performed to evaluate knee joint AC destruction in mice receiving AAV5-GFP (control) or AAV5-miR-204 injection. n = 5. Mann−Whitney test. d Intraarticular injection of AAV5-miR-204 reduces pain sensitivity induced by OA. The von Frey test was performed in 9-month-old mice receiving AAV injection at the age of 3 months. n = 6. e IHC results of Runx2, Adamts5, and MMP13 expression in osteoarthritic joints receiving AAV injection. Red arrowheads, IHC-positive cells. Scale bar, 50 μm. f−h Quantification of positive IHC signals per mm² of knee joints for detection of Runx2 (f), Adamtas5 (g), and MMP13 (h). n = 3. Unpaired Student’s t test. *P < 0.05, **P < 0.01, ***P < 0.001