Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jun 28;10:2864. doi: 10.1038/s41467-019-10891-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 5 — GMAP210 captures vesicles carrying VAMP7. a Confocal images showing the relative localization of VAMP7 (green) and CTR433 (red) in Jurkat cells expressing control (ShC) or GMAP210 specific shRNA (Sh3, Sh8) (GMAP210, nucleus in gray). Dot plot on the right side of the panel show the quantification of the percentage of CTR433 (Golgi marker) overlapping with VAMP7 (Manderson coefficient). Scale bar 5 μm. Each dot = one cell; horizontal lines = median. ****P < 0.0001 (one-way ANOVA). b Confocal images showing the localization of VAMP7 or VAMP3 (red) in Jurkat cells expressing a GFP-GMAP210-ActA chimera (GFP-GMAP210-Mit) or a GFP-ActA chimera (GFP-Mit), treated for 4 h with nocodazol (5 μg/mL) (nucleus in blue and mitochondria in Gray). Dot plot on the right side of the panel shows the quantification of the percentage of VAMPs (VAMP7 or VAMP3) overlapping with the GFP staining (GFP-mit or GFP-GMAP210-mit, Manderson coefficient). Scale bar 5 μm. Each dot = one cell; horizontal lines = median. ****P < 0.0001, ns: non-significant (one-way ANOVA). Data and Images represent two independent experiments in a and b