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A. Fab alone, 4X Fab + 4-arm PEG and 8X Fab + 8-arm PEG conjugates all exhibited very similar behavior in the TR-FRET assay on a milligram of Fab basis. B. Visco-elastic behavior of 8X Fab + 8-arm and 4X Fab-4-arm PEG conjugates were compared at a sheer rate of 1/1000 seconds, in PBS at 25°C. The 8-arm formulation is significantly lower in viscosity than the 4X Fab + 4-arm PEG, at Fab concentrations above 70 mg/mL, as seen in the zoomed-in view.
