Table 5. Mitochondrial swelling: Effect of successive addition of Ca2+.
Calcium | Absorbance at 520 nm | ||
---|---|---|---|
Sham | CL | UUO | |
Contaminant Ca2+ | 0.70 ± 0.12a | 0.39 ± 0.02b | 0.28 ± 0.03b |
Additional Ca2+: 5 μM |
0.61 ± 0.10a | 0.34 ± 0.02b | 0.26 ± 0.03b |
10 μM | 0.58 ± 0.10a | 0.31 ± 0.02a, b | 0.26 ± 0.05b |
20 μM | 0.57 ± 0.10a | 0.31 ± 0.02a, b | 0.25 ± 0.04b |
50 μM | 0.56 ± 0.10a | 0.29 ± 0.01b | 0.24 ± 0.04b |
100 μM | 0.55 ± 0.09a | 0.29 ± 0.01b | 0.24 ± 0.04b |
Means ± SEM of 3 assays (each group) carried out with different mitochondrial preparations. Different lower case letters as superscripts after SEM indicate statistical differences among the Ca2+ concentration-matched mean values (p<0.05; one-way ANOVA followed by Tukey´s test).