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. 2019 Jun 28;9:9410. doi: 10.1038/s41598-019-45153-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Total bacterial load quantification by qPCR corresponding to the total number of 16S rRNA encoding gene copies per mg of intracaecal content of mice subjected to 2G (n = 10) or 3G (n = 11) hypergravity and 1G control mice (n = 7). Statistical analyses were done using the Mann-Whitney U test. The upper and lower ranges of the box represent the 75% and 25% quartiles, respectively. Error bars reflect standard error of the mean.