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. 2019 Jun 28;9:9410. doi: 10.1038/s41598-019-45153-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Intracaecal 16S rDNA gene-based real-time PCR quantification of the relative abundance of Parabacteroides distasonis, Paraprevotella clara, Parasutterella excrementihominis, and Flavonifractor plautii. Data are expressed as the relative proportion of the number of 16S rDNA copies of each target to the total number of 16S rDNA copies per mg of intracaecal content of mice subjected to 2G (n = 10) or 3G (n = 11) hypergravity and 1G control mice (n = 7). Statistical analyses were done using the Mann-Whitney U test. *p < 0.05; **p < 0.0005. Error bars reflect standard error of the mean.