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. 2019 Jun 28;14(6):e0199653. doi: 10.1371/journal.pone.0199653

Fig 4.

Fig 4

a. SDS-PAGE gel of SV Nedd5 expression devices with different N-terminal tags. M = protein molecular weight marker; (-) = Uninduced sample; (+) Induced samples. SV denotes expression from SureVector assembled plasmids, ptac denotes expression from the commercially available ptac based plasmids. Green stars denote expressed N-terminal tagged Nedd5-fusion proteins. Fig 4b. SDS-PAGE Gel of SV Nedd5 Expression Devices with Different C-Terminal Tags. M = protein molecular weight marker; (-) = Uninduced samples; (+) = Induced samples. SV denotes expression from SureVector assembled plasmids, ptac denotes expression from the commercially available ptac based plasmids. Green stars denote expressed N-terminal tagged Nedd5-fusion proteins.