Fig 8. DMRL synthesis rates.

DMRL synthesis rates of systems K6A7, K7A6, K8A9, K9A8 and negative control K5A5 were obtained by inoculating single colonies into 5 ml of LB-kan-amp media followed by overnight incubation at 37°C with shaking at 250 rpm. One ml of these starter cultures was inoculated into separate 250 ml flasks each containing 49 ml of LB-kan-amp. Incubation at 37°C with shaking at 250 rpm continued until OD₆₀₀ values reached between 0.3 and 0.5. IPTG was added to a final concentration of 0.5 mM and incubation continued. At regular intervals, 1.0 ml samples were retrieved from each culture and OD₆₀₀ values measured. Samples were then centrifuged to remove cells and OD₄₀₉ values of supernatants obtained. OD₄₀₉ values were normalized by dividing by the OD₆₀₀ values and these numbers plotted as a function of OD₄₀₉ post-IPTG addition.