Table 3. Combinations of Table 2 functional and control devices used to re-create the DMRL biosynthetic pathway system.

Transformations into BL21(GOLD)
System		rib Genes Expressed	Rib genes Missing	Plate on	Assay
Device 1	Device 2
K1	-	A	B, D and E	Kan	Control
K2	-	B	A, D and E	Kan	Control
K3	-	D	A, B and E	Kan	Control
K4	-	E	A, B and D	Kan	Control
K5	-	-	A, B, D and E	Kan	Control
K6	-	A and D	B and E	Kan	Control
K7	-	B and E	A and D	Kan	Control
K8	-	A and E	B and D	Kan	Control
K9	-	B and D	A and E	Kan	Control
A1	-	A	B, D and E	Amp	Control
A2	-	B	A, D and E	Amp	Control
A3	-	D	A, B and E	Amp	Control
A4	-	E	A, B and D	Amp	Control
A6	-	A and D	B and E	Amp	Control
A7	-	B and E	A and D	Amp	Control
A8	-	A and E	B and D	Amp	Control
A9	-	B and D	A and E	Amp	Control
A5	-	-	A, B, D and E	Amp	Control
K1	A9	A, B and D	E	Kan+Amp	Control
K2	A8	B, A and E	D	Kan+Amp	Control
K3	A8	D, A and E	B	Kan+Amp	Control
K4	A9	E, B and D	A	Kan+Amp	Control
K6	A7	A, D, B and E	-	Kan+Amp	Test
K7	A6	B, E, A and D	-	Kan+Amp	Test
K8	A9	A, E, B and D	-	Kan+Amp	Test
K9	A8	B, D, A and E	-	Kan+Amp	Test