Fig. 4.

Seed-point extraction segmentation step and all-in-one segmentation approaches. a Results of segmentation, representative image of rDIC-Koos-reconstructed DIC image followed by foreground-background segmentation with Traniable Weka Segmentation. Blue points indicate seeds based on which cells are segmented using marker-controlled watershed. Note absence of seed-points for “all-in-one” segmentation approaches. b Dependency between number of cells used for training and Dice coefficient for Celldetect